Triple Sugar Iron Agar Dehydrated Culture Media

The medium’s core strength is its powerful, multi-functional differentiation system. It contains dextrose, lactose, and sucrose as fermentable carbohydrates, with Phenol Red as a pH indicator to reveal acid production (yellow color). For gas and H₂S production, it includes iron salts and Sodium Thiosulfate. This four-way differentiation—acid/alkaline slant, butt reaction, gas production, and blackening due to H₂S—allows for the rapid presumptive identification of enteric organisms. Its formula is a time-tested modification used extensively in diagnostic microbiology.

Please note: This medium should be used in conjunction with a urease test to eliminate Proteus spp. when screening for Salmonella spp. It is not intended for use in the diagnosis of disease or other conditions in humans.

Features:

• Used for the differentiation of microorganisms based on dextrose, lactose, and sucrose fermentation
• System detects hydrogen sulfide (H₂S) production (blackening of the medium)
• Contains Phenol Red as an indicator to reveal acid production from fermentation
• Recommended for differentiation of enteric, Gram-negative bacilli from clinical and food samples
• Formula is a classic modification that simplifies enteric organism identification

Specifications:

• Brand: Neogen® Culture Media
• Analyte: Enterobacteriaceae
• Platform: Dehydrated Culture Media
• Mass: 500 g
• Grams per Liter: 65.00
• Preparation: Suspend 65 g of the medium in one liter of purified water, heat to dissolve, and autoclave at 121°C for 15 minutes (inferred)

Cross Reference:

• Acumedia: Triple Sugar Iron Agar (7162)
• BD / Difco: 226540
• BD / BBL: N/A
• Oxoid: CM0277
• Merck: 1.03915