Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar Dehydrated Culture Media

The agar’s core strength is its powerful dual selective and differential system. Bile Salts and increased salt concentrations inhibit most non-Vibrio species. For differentiation, Sucrose is included as a fermentable carbohydrate, and the pH indicators Bromothymol Blue and Thymol Blue provide a distinct color reaction: Sucrose-fermenting Vibrios (e.g., V. cholerae) produce acid, resulting in yellow colonies.

Please note: TCBS Agar must NOT be autoclaved. It is not intended for use in the diagnosis of disease or other conditions in humans.

Features:

• Highly selective for the isolation of Vibrio cholerae and other enteropathogenic Vibrios
• Contains high salt and Bile Salts to inhibit non-Vibrio bacteria
• Sucrose fermentation is differentiated by Bromothymol and Thymol Blue (yellow colonies for fermenters)
• Conforms to ISO 21872 standards for food testing
• Meets the nutritional and halophilic requirements of Vibrio spp
• Requires pre-incubation in Alkaline Saline Peptone Water (ASPW) according to ISO 21872-2

Specifications:

• Brand: Neogen® Culture Media
• Analyte: Vibrio spp
• Platform: Dehydrated Culture Media
• Mass: 500 g
• Preparation: Suspend 88 g of the medium in one liter of purified water, heat to dissolve, and DO NOT AUTOCLAVE

Cross Reference:

• Acumedia: TCBS Agar (7210)
• BD / Difco: 265020
• BD / BBL: N/A
• Oxoid: CM0333
• Merck: 1.10263