PPLO Broth Base without Crystal Violet Dehydrated Culture Media

The broth base is fortified with a rich blend of nutrients from Heart Infusion and Yeast Enriched Peptone, providing a comprehensive source of amino acids and vitamins for robust growth. As a base, it requires the aseptic addition of an appropriate sterile serum supplement to become a complete medium. This combination of a highly specific base and a tailored supplement provides the perfect platform for the isolation and cultivation of Mycoplasma spp.

Please note: This product is an agar base and requires supplementation. It is not intended for use in the diagnosis of disease or other conditions in humans.

Features:

• Used for the isolation and cultivation of Mycoplasma spp.
• Formula is without Crystal Violet, as it may inhibit the growth of Mycoplasma
• Nutrient-rich base with Heart Infusion and Yeast Enriched Peptone
• Requires the aseptic addition of a sterile serum supplement for a complete medium
• Based on a scientifically validated formula by Morton and Lecce

Specifications:

• Brand: Neogen® Culture Media
• Analyte: Mycoplasma spp.
• Platform: Dehydrated Culture Media
• Mass: 500 g
• Preparation: Dissolve 21 g of the medium in 100 mL of purified water, autoclave at 121°C for 15 minutes, cool to 45-50°C, and aseptically add 300 mL of an appropriate sterile serum supplement

Cross Reference:

• Acumedia: PPLO Broth w/o CV (7485)
• BD / Difco: 255420
• BD / BBL: 211458
• Oxoid: CM0403*
• Merck: N/A

* Similar formula, but not exact.