UVM Modified Listeria Enrichment Broth Dehydrated Culture Media

The medium's strength lies in its comprehensive selective and differential system. Selective agents such as Nalidixic Acid (inhibits Gram-negative organisms) and Acriflavin (inhibits Gram-positive bacteria) work together to suppress competing flora. For differentiation, Esculin is included, which is hydrolyzed by Listeria spp. to provide a distinct reaction (typically indicated by blackening in the presence of ferric ions, though not explicitly listed as an additive in the base). The high salt tolerance of Listeria is utilized to further inhibit Enterococci. This robust formulation ensures high recovery rates and reliable results across a wide range of food and environmental samples.

Please note: This product is a broth base and may require an additional supplement (such as ferric ammonium citrate) for the differentiation reaction to be visible. It is not intended for use in the diagnosis of disease or other conditions in humans.

Features:

• Used for the selective enrichment of Listeria spp. from food and clinical specimens
• Contains Nalidixic Acid and Acriflavin to inhibit competing Gram-negative and Gram-positive bacteria
• Esculin is included for differentiation based on hydrolysis
• Utilizes the high salt tolerance of Listeria to aid in selectivity
• Nutrient-rich base of enzymatic digests and extracts supports robust growth
• Suitable for Listeria growth over a wide pH range (5.0–9.6)

Specifications:

• Brand: Neogen® Culture Media
• Analyte: Listeria spp.
• Platform: Dehydrated Culture Media
• Mass: 500 g
• Grams per Liter: 52.00
• Preparation: Suspend 52 g of the medium in one liter of purified water and autoclave at 121°C for 15 minutes (inferred)

Cross Reference:

• Acumedia: UVM Mod Listeria Enrich Broth (7409)
• BD / Difco: 222330
• BD / BBL: N/A
• Oxoid: CM0863+
• Merck: 1.10824