PALCAM Broth Dehydrated Culture Media

The broth's unique formula is based on the work of Van Netten et al., providing a superior blend of selectivity and differentiation. Lithium Chloride is a key selective agent that helps inhibit competing organisms. A double indicator system of esculin hydrolysis and mannitol fermentation allows for clear differentiation: a blackening of the medium from esculin hydrolysis and a color change from the Phenol Red indicator provide an unmistakable sign of a positive Listeria presence. This medium requires the aseptic addition of a Palcam PAC Supplement to become a complete medium.

Please note: This product is not intended for use in the diagnosis of disease or other conditions in humans.

Features:

• Used for the selective enrichment of Listeria spp. from food and environmental samples
• Contains Lithium Chloride and other selective agents to inhibit competing microorganisms
• Double indicator system of esculin hydrolysis and mannitol fermentation aids in differentiation
• Ideal for testing a variety of food samples, including raw milk
• Requires the aseptic addition of a Palcam PAC Supplement for a complete medium

Specifications:

• Brand: Neogen® Culture Media
• Analyte: Listeria spp.
• Platform: Dehydrated Culture Media
• Mass: 500 g
• Formula (per liter): Peptone (23.0 g), Yeast Extract (5.0 g), Lithium Chloride (10.0 g), Esculin (0.8 g), Ferric Ammonium Citrate (0.5 g), Mannitol (5.0 g), Phenol Red (0.08 g), Lecithin (1.0 g), Polysorbate 80 (2.0 g)
• Preparation: Suspend 47 g of medium in one liter of purified water, autoclave, cool to 45-50°C, and aseptically add Palcam PAC Supplement

Cross Reference:

• Acumedia: PALCAM Broth (7670)
• BD / Difco: N/A
• BD / BBL: N/A
• Oxoid: N/A
• Merck: 1.10823+

+ Supplement required.