Mannitol Salt Agar Dehydrated Culture Media

The medium's dual functionality is its key advantage. A high salt concentration (7.5%) acts as a powerful selective agent, effectively inhibiting the growth of most bacteria other than staphylococci. For differentiation, D-Mannitol is included as a fermentable carbohydrate and Phenol Red as a pH indicator. Pathogenic staphylococci ferment the mannitol, producing acid which turns the medium a distinct yellow color. Non-pathogenic strains do not ferment mannitol, and their colonies remain red. The medium conforms to Harmonized USP/EP/JP Requirements, ensuring its reliability for compliance-critical applications.

Please note: Mannitol Salt Agar is for laboratory use in the isolation of staphylococci and is not intended for use in the diagnosis of disease or other conditions in humans.

Features:

• Used for the selective isolation of staphylococci
• High salt concentration (7.5% NaCl) inhibits most other bacteria
• Differentiates pathogenic staphylococci from non-pathogenic strains
• Mannitol fermentation produces a yellow color change for clear identification
• Conforms to Harmonized USP/EP/JP Requirements
• Ideal for samples from heavily contaminated sources, cosmetics, and microbial limit tests

Specifications:

• Brand: Neogen® Culture Media
• Analyte: Staphylococcus aureus
• Platform: Dehydrated Culture Media
• Mass: 500 g
• Formula (per liter): Enzymatic Digest of Casein (5.0 g), Enzymatic Digest of Animal Tissue (5.0 g), Beef Extract (1.0 g), D-Mannitol (10.0 g), Sodium Chloride (75.0 g), Phenol Red (0.025 g), Agar (15.0 g)
• Preparation: Suspend 111 g of the medium in one liter of purified water, heat to dissolve, and autoclave at 121°C for 15 minutes

Cross Reference:

• Acumedia: Mannitol Salt Agar (7143)
• BD / Difco: 230650
• BD / BBL: 211407
• Oxoid: CM0085
• Merck: 1.05404