MacConkey Broth Dehydrated Culture Media

The medium's selective power comes from Ox bile, which suppresses the growth of most Gram-positive bacteria. The primary differential characteristic is the fermentation of lactose, which is indicated by the Bromocresol Purple dye. A positive result is a distinct color change from purple to yellow, which is an unmistakable sign of coliform presence. The broth conforms to Harmonized USP/EP/JP Requirements, ensuring its reliability for compliance-critical applications.

Please note: MacConkey Broth is for laboratory use in the detection of coliform bacteria and is not intended for use in the diagnosis of disease or other conditions in humans.

Features:

• Used for the selective enrichment of coliform bacteria in milk and water
• Ox bile acts as a selective agent, inhibiting most Gram-positive organisms
• Lactose fermentation results in a color change from purple to yellow
• Conforms to Harmonized USP/EP/JP Requirements
• Preparation includes inverted Durham tubes for gas detection

Specifications:

• Brand: Neogen® Culture Media
• Analyte: E. coli
• Platform: Dehydrated Culture Media
• Mass: 500 g
• Formula (per liter): Pancreatic Digest of Gelatin (20.0 g), Lactose Monohydrate (10.0 g), Dehydrated Ox Bile (5.0 g), Bromocresol Purple (0.01 g)
• Preparation: Dissolve 35 g of the medium in one liter of purified water, mix, dispense into tubes with Durham tubes, and autoclave at 121°C for 15 minutes

Cross Reference:

• Acumedia: MacConkey Broth (7185)
• BD / Difco: 220100
• BD / BBL: N/A
• Oxoid: CM0505
• Merck: 1.05396