m-Endo Agar Dehydrated Culture Media

The medium's unique formula, prepared according to the APHA and US EPA standards, is both selective and differential. The presence of Sodium Deoxycholate and Sodium Lauryl Sulfate inhibits the growth of unwanted Gram-positive bacteria, while the fermentation of lactose by coliforms results in a characteristic metallic sheen. This distinct visual indicator simplifies presumptive identification. m-Endo Agar is often used as the second step in a two-step membrane filtration procedure, following enrichment in Lauryl Tryptose Broth for higher coliform counts.

Please note: This product must NOT be autoclaved and requires the addition of non-denatured Ethanol during preparation. It is not intended for use in the diagnosis of disease or other conditions in humans.

Features:

• Used for the enumeration of coliforms in water by the membrane filtration method
• Prepared according to APHA and US EPA formulas
• Lactose fermentation results in a metallic sheen for presumptive identification
• Contains selective agents to inhibit unwanted bacteria
• Ideal for assessing water quality and monitoring disinfection effectiveness

Specifications:

• Brand: Neogen® Culture Media
• Analyte: Coliforms
• Platform: Dehydrated Culture Media
• Mass: 500 g
• Formula (per liter): Lactose (9.4 g), Tryptose (7.5 g), Enzymatic Digest of Casein (3.7 g), Enzymatic Digest of Animal Tissue (3.7 g), Sodium Chloride (3.7 g), Potassium Phosphate, Dibasic (3.3 g), Sodium Sulfite (1.6 g), Yeast Extract (1.2 g), Potassium Phosphate, Monobasic (1.0 g), Basic Fuchsin (0.8 g), Sodium Deoxycholate (0.1 g), Sodium Lauryl Sulfate (0.05 g), Agar (15.0 g)
• Preparation: Suspend 51 g in one liter of purified water with 20 mL of non-denatured Ethanol, heat to dissolve, and cool (DO NOT AUTOCLAVE)

Cross Reference:

• Acumedia: m-Endo Agar (7724)
• BD / Difco: 273620
• BD / BBL: N/A
• Oxoid: CM0479
• Merck: 1.11277