Carbol-Fuchsin Solution (Kinyoun)

The solution’s primary benefit is its precise formulation, which allows for staining without the application of heat (the cold method), improving safety and workflow convenience. Carbol-Fuchsin is a powerful basic dye that binds strongly to the high-mycolic acid content of the bacterial cell wall, which resists decolorization by acid-alcohol, resulting in the characteristic bright red/pink staining of acid-fast bacilli. Choosing this ready-to-use formulation eliminates hazardous and time-consuming in-house preparation, guaranteeing consistent staining intensity every time.

Features:

• Enhanced Safety: By removing the requirement for Bunsen burners or hot plates during the staining process, the Kinyoun method reduces the inhalation of phenolic vapors and prevents accidental fire hazards.
• Workflow Efficiency: Pre-mixed and ready to use, this solution saves valuable technician time and guarantees lot-to-lot consistency for clinical and research diagnostics.
• Superior Penetration: The high phenol content acts as a powerful solvent, ensuring the basic fuchsin dye binds strongly to the lipid-rich cell walls of mycobacteria.
• Reliable Diagnostics: Specifically formulated to meet the rigorous requirements of tuberculosis screening and environmental microbiology.

Laboratory Protocol & Safety:

• Chemical Hazard: This product contains phenol and basic fuchsin. It is known to the State of California to cause cancer and reproductive harm (Prop 65). Always handle in a well-ventilated area or fume hood using appropriate PPE (gloves, goggles, and lab coat).
• Decolorization: After staining with Carbol-Fuchsin, ensure the slide is treated with Acid-Alcohol until the run-off is clear. Only acid-fast organisms will retain the primary pink stain.
• Storage: Store at room temperature (15°C to 30°C) and keep the bottle tightly closed. Protect from excessive light to prevent the degradation of the basic fuchsin dye.