MI-Broth Media Liquid Media Ampoules

The medium's primary benefit is its ability to provide two simultaneous results. Total coliforms are detected by the production of b-galactosidase, which cleaves the substrate MUGal to produce a compound that fluoresces on exposure to UV light. Non-coliforms do not produce this enzyme and therefore do not fluoresce on the medium. For specific E. coli identification, the medium contains IBDG. The b-glucuronidase produced by E. coli cleaves this substrate to create a distinctive blue indigo color in the colonies. As E. coli is also a total coliform and also produces b-galactosidase, it will also fluoresce. For added security, the antibiotic Cefsulodin is present to inhibit the growth of Gram-positive bacteria and some non-coliform Gram-negative bacteria that can cause false positive reactions. All of this functionality is contained within individual, pre-portioned 2 mL ampoules, which undergo detailed quality control checks to guarantee uniform and stable sterile media every time.

Features:

• Dual-detection method for total coliforms and Escherichia coli
• Fluorogenic detection for coliforms using the MUGal substrate
• Chromogenic detection for E. coli using the IBDG substrate, producing blue colonies
• Cefsulodin inhibits Gram-positive and non-coliform Gram-negative bacteria
• Packaged in individual, pre-portioned 2 mL ampoules for ease of use and consistency
• Undergoes detailed quality control checks for uniform preparation and sterility

Characteristics:

• Microbiological analysis for: Total coliforms and E. coli
• Suitable for: Potable water, beer and wine, waste water, soft drinks and concentrates, fruit juices, fermented products