Accuris One-Step RT-qPCR Kits

The optimized buffer system includes powerful RNase inhibitors and a thermostable MMLV-derived reverse transcriptase for robust first-strand cDNA synthesis. Accuris Hot Start Taq Polymerase uses an antibody-mediated activation mechanism, enabling room temperature setup while reducing non-specific amplification. An inert blue tracking dye is included to improve pipetting accuracy and minimize handling errors.

Three kit options are available to meet diverse experimental needs:

• qMAX™ Green One Step Kits - Incorporate Accuris' proprietary intercalating dye, delivering higher fluorescence and lower PCR inhibition than conventional dyes such as SYBR
• qMAX™ Probe One Step Kits - Optimized for probe-based detection with TaqMan™, Scorpions®, and molecular beacon chemistries
• qMAX™ Probe One Step Multiplex Kits - Specifically designed for simultaneous detection of multiple RNA targets, with balanced buffer chemistry for complex assays including low-copy viral RNA

All Accuris One Step Kits are compatible with both fast and standard cycling protocols and are available in high, low, or no ROX formulations for broad qPCR instrument compatibility. With enhanced sensitivity, speed, and reproducibility, these kits provide exceptional performance for research and clinical applications.

Features:

• Highly sensitive one-step RT-qPCR directly from RNA templates
• Ready-to-use 2X master mix and 20X reverse transcriptase for simplified workflows
• Thermostable MMLV-derived reverse transcriptase for robust cDNA synthesis
• Accuris Hot Start Taq Polymerase reduces non-specific amplification
• Inert blue tracking dye improves pipetting accuracy and reduces error
• Available in Green, Probe, and Multiplex formats for flexible assay design
• Multiplex kits optimized for complex RNA samples and low-copy viral detection
• Compatible with standard and fast cycling protocols
• Offered in high, low, or no ROX formulations for instrument compatibility