Netwell™ Polyester Membrane Inserts

Netwell™ inserts are the gold standard for culturing small organs, tissue slices, or explants at the air-media interface. The mesh bottom allows nutrients to reach the tissue from the media below while providing the structural support necessary for growth. Beyond culture, they are excellent for the coarse filtration of tissue homogenates, cell suspensions, and microcarriers.

Features:

• Air-Media Interface Optimized: Ideal for organotypic cultures where tissues require both atmospheric exposure and nutrient access from the basal side
• Precision PET Mesh: Available in two mesh sizes (74 µm or 440 µm) to accommodate various tissue types and filtration needs
• Ready-to-Use: Supplied sterile and pre-loaded into standard 12-well microplates for immediate laboratory use
• Versatile Utility: Functions as a cell strainer, tissue carrier for immunohistochemical staining, or a 3D growth support
• Compatibility: Designed to work seamlessly with Netwell™ carrier kits for batch processing of up to 12 samples at once

Application Recommendations:

• 74 µm Mesh: Best for fine cell suspensions, microcarriers, and small tissue fragments
• 440 µm Mesh: Ideal for larger tissue slices, organ explants, and coarse filtration of homogenates
• Staining: Use these inserts as carriers to move tissue slices through sequential staining reagents without ever needing to touch the sample with forceps