Ultra-Low Attachment (ULA) Cell Culture Flasks

By neutralizing surface signals, the ULA surface minimizes protein adsorption and spontaneous cellular activation. This ensures that stem cells remain in their undifferentiated state and cancer cells maintain their colony-forming potential. Each flask features a vent cap with a 0.2 µm hydrophobic membrane, providing continuous, sterile gas exchange while minimizing the risk of contamination.

Features:

• Advanced Hydrogel Surface: A hydrophilic, neutrally charged layer inhibits cell attachment and protein binding
• Optimized for 3D Growth: Promotes the self-assembly of uniform 3D spheroids and neurospheres by forcing cell-to-cell rather than cell-to-surface interaction
• Superior Contamination Protection: Equipped with vent caps for consistent CO₂ exchange and canted necks for easy pipette access with reduced splash-back
• Exceptional Clarity: Manufactured from optically clear virgin polystyrene for effortless microscopic monitoring of suspended clusters
• Rigorous Quality Control: 100% integrity tested, lot traceable, gamma-irradiated, and certified nonpyrogenic
• Closed System Safety: Flasks provide a more secure environment than open dishes for long-term expansion of sensitive cell lines

Primary Applications:

• Stem Cell Expansion: Culturing embryoid bodies (EBs) or mesenchymal stem cells in a suspended, undifferentiated state
• Oncology: Enrichment of cancer stem cells and large-scale tumor spheroid production for drug screening
• Immunology: Maintenance of primary leukocytes and non-activated immune cells
• Organoid Research: Scalable maintenance of organoid-precursor suspensions.

Tip: U-Shape Design (3814): The 75 cm² flask features a U-shaped design that eliminates "dead corners," improving media circulation and making it easier to recover cells during harvesting.