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CELLine Bioreactors

from $190.66

Description

In an effort to push science forward, Wheaton is manufacturing a new line of membrane-driven flasks for high density cell culture. The flasks are designed to enhance small scale bio-production for antibody and protein generation. Conventional in vivo or in vitro cell culture methods can be laborious, may have low cell density and require significant purification. CELLine flasks address these three areas of limitation observed in static tissue culture flasks.

Handling Requirements: CELLine flasks reduce the handling requirement by requiring less consumable items and allowing longer run times due to their unique metabolite regulating upper membrane. This membrane allows for bulk media storage during operation to ensure constant and regulated nutrient access for the cells.

Cell Densities: CELLine flasks ensure maximum gas exchange by placing the gas permeable lower membrane directly next to the cells. This allows for optimal oxygen and carbon dioxide transfer for metabolizing cells.

Purification: The upper and lower membranes form an optimized compartment for cell proliferation. This allows for the reduced use of growth factors and hormones and concentrates the antibody and proteins of interest.

Benefits
  • • Disposable and ready-to-use
  • • High cell density and high product concentration
  • • Reduces operation time
  • • Decreases use of consumables
  • • Cost-efficient, space saving, and stackable
  • • No additional equipment required for operation

How does the CELLine Flask Work?


Media Compartment: The media compartment allows for bulk storage of cell culture growth medium. This reduces the media refreshing requirement significantly as the media compartment is fifty times the size of the cell compartment.

Metabolite Regulating Upper Membrane: The upper dialysis membrane has a 10 kDa cut off limit. This regulates the flow of metabolites to and from the cell compartment and retains all proteins in the cell compartment.

Cell Compartment: The cell compartment provides the ideal area to inoculate and achieve high density cultures. The compartment concentrates cells, their products, and limits the requirement for any exogenous growth factors.

Gas Permeable Lower Membrane: With static cultures, gas transfer rates can be the limiting factor in high density cultures. The CELLine flask places the cells directly against the gas permeable membrane to achieve optimal levels of oxygen and carbon dioxide.
 
CELLine Diagram

 

 

Seeding and Harvesting Densities CELLine 350 CELLine 1000
Preculture (Viable Cells) 8 x 106 25 x 106
Inoculation Volume 5 mL 15 mL
Inoculation Concentration 1.5 x 106 Viable Cells/mL
Harvesting Concentration 107 to 108
Titer 1 to 5 mg/ML
Antibody Yield per Month 10 to 100 mg 30 to 1000 mg

Type your desired quantities in the fields below, then click:
Price Chart
Cat Code Description Type QuantityPrice Add Quantity
WCL1000-1 CELLine 1000 Bioreactor Flask 1000ml media compartment 15 ml cell growth compartment for antibody and protein production Suspension Each$240.60
WCL1000-3 CELLine 1000 Bioreactor Flask 1000ml media compartment 15 ml cell growth compartment for antibody and protein production Suspension Case of 3$709.31
WCL1000AD-1 CELLine AD 1000 Bioreactor Flask 1000ml media compartment 15 ml cell growth compartment for antibody and protein production Adherent Each$242.85
WCL1000AD-3 CELLine AD 1000 Bioreactor Flask 1000ml media compartment 15 ml cell growth compartment for antibody and protein production Adherent Case of 3$720.66
WCL0350-1 CELLine 350 Bioreactor Flask 350ml media compartment 5 ml cell growth compartment for antibody and protein production Suspension Each$190.66
WCL0350-5 CELLine 350 Bioreactor Flask 350ml media compartment 5 ml cell growth compartment for antibody and protein production Suspension Case of 5$930.61
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