Intended Use
Letheen Broth Base is used with Tween 80 (Polysorbate 80) for the testing of quaternary ammonium compounds for antimicrobial activity.
Product Summary and Explanation
In 1948, Weber and Black described the value of a highly nutritional solid medium containing neutralizing agents for quaternary ammonium compounds in sanitizers. The addition of Lecithin and Polysorbate 80 to Tryptone Glucose Extract (TGE) Agar resulted in a medium that effectively neutralizes quaternary ammonium compounds while testing germicidal activity. Letheen Agar is a modification of TGE Agar with the addition of Lecithin and Polysorbate 80.
Letheen Broth Base was developed as a subculture medium for the neutralization of quaternary ammonium compounds in disinfectant testing. Quisno, Gibby, and Foter discovered that adding Lecithin and Polysorbate 80 to F.D.A. Broth resulted in a medium that neutralized high concentrations of quaternary ammonium salts. The resulting medium, termed “Letheen” (a combination of Lecithin and Tween) was easy to prepare and clear in appearance, aiding to visual inspection for growth. Letheen Broth Base is recommended by the Official Methods of Analysis of the Association of Official Analytical Chemists (AOAC) for use with disinfectants containing cationic surface active materials.
Letheen Broth Base is specified for use by the American Society for Testing Materials (ASTM) in Standard Test Method for Preservatives in Water-Containing Cosmetics. Total neutralization of disinfectants is critical. Disinfectant residues can result in a false negative (no-growth) test.
Choose sharing option
×
Email
URL
WhatsApp
Request A Quote
Thank you!
We have received your request and are working hard on your quotation. Please allow 24 - 48 business hours for quote processing. You will receive a copy of your request to the email attached.
We look forward to serving you.
Thank you!
We have received your request and are working hard on your quotation. Please allow 24 - 48 business hours for quote processing. You will receive a copy of your request to the email attached.
We look forward to serving you.
The Lab Depot uses cookies to improve your experience on our site. By clicking “Dismiss”, you consent to the use of all cookies which enable you to have a more personalized shopping experience. The Lab Depot does not sell any personal information. For more information, please read our privacy policy.