Demi-Fraser Broth Base
Starting at $78.91
|Acumedia||BD / Difco||BD / BBL||Oxoid||Merck|
|Demi-Fraser Broth Base (7656)||265320||N/A||CM1053||1.10398+|
+ Supplement required.
Demi-Fraser Broth Base is used with ferric ammonium citrate for the selective enrichment of Listeria species.
Product Summary and Explanation
Listeria monocytogenes, described in 1926 by Murray, Webb, and Swann, is a widespread problem in public health and food industries. This organism has the ability to cause human illness and death, particularly in immunocompromised individuals and susceptible pregnant women. Epidemiological evidence from outbreaks of listeriosis indicate the principle route of transmission is via the consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese and pate. Listeria species are ubiquitous in nature, present in a wide range of unprocessed foods and in soil, sewage, and river waste.
Demi-Fraser Broth Base is a modification of Fraser Broth Base, developed by Fraser and Sperber, and used for the rapid detection of Listeria from food and environmental samples. In Demi-Fraser Broth Base, the nalidixic acid and acriflavine concentration have been reduced in accordance with AFNOR guidelines. Listeria species grow over a pH range of 5.0 - 9.6, and can survive in food products with pH levels outside these parameters. Identification of Listeria is based on successful isolation of the organism, biochemical characterization, and serological confirmation.