Violet Red Bile Agar w/MUG
Manufacturer: Neogen Inc.
Starting at from $299.20 Sale $284.24
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Violet Red Bile Agar w/MUG
Description
Acumedia | BD / Difco | BD / BBL | Oxoid | Merck |
Violet Red Bile Ag w/ MUG (7359) | 229100 | N/A | CM0978 | 1.0403 |
Intended Use
Violet Red Bile Agar W/ MUG is used for the detection of coliforms and the fluorogenic detection of Escherichia coli.
Product Summary and Explanation
Violet Red Bile Agar W/ MUG is specified in many procedures to enumerate coliforms in food and dairy products. In 1982, Feng and Hartman developed a rapid fluorogenic assay for Escherichia coli by incorporating 4-methylumbelliferyl-β-D-glucuronide (MUG) into Lauryl Tryptose Broth. Incorporating MUG into Violet Red Bile Agar permits the detection of E. coli among the coliform colonies. Standard methods procedures specify Violet Red Bile Agar with MUG for detecting E. coli in food and dairy products by fluorescence. This medium is often abbreviated as VRBA w/ MUG.
Principles of the Procedure
Enzymatic Digest of Gelatin is the source of carbon, nitrogen, vitamins, and minerals in Violet Red Bile Agar w/ MUG. Yeast Extract supplies B-complex vitamins that stimulate bacterial growth. Bile Salts Mixture and Crystal Violet inhibit Gram-positive bacteria. Lactose is a carbohydrate source. Neutral Red is a pH indicator. MUG (4-methylumbelliferyl-β-D-glucuronide) is a substrate used for detecting glucuronidase activity. Agar is the solidifying agent.