LB Agar, Lennox
Starting at $855.69
|Acumedia||BD / Difco||BD / BBL||Oxoid||Merck|
|LB Agar, Lennox (7289)||240110||N/A||N/A||N/A|
LB Agar, Lennox is used in molecular genetic studies.
Product Summary and Explanation
LB Agar is nutritionally rich, developed by Lennox for growth and maintenance of pure cultures of recombinant strains of E. coli. These strains are generally derived from E. coli K12, which are deficient in B vitamin production. This strain of E. coli has been further modified through specific mutation to create an auxotrophic strain that is not capable of growth on nutritionally deficient media. LB Agar provides all nutritional requirements of these organisms. LB Agar contains half the sodium chloride level of the Miller formulation of LB Agar, allowing the researcher to select the optimal salt concentration for a specific strain.
Principles of the Procedure
The nitrogen, amino acids, and carbon sources are provided by Enzymatic Digest of Casein. Vitamins and certain trace elements are supplied by Yeast Extract. Sodium ions for transport and osmotic balance are provided by Sodium Chloride. Agar is the solidifying agent.