Brain-Heart Infusion Broth
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|Acumedia||BD / Difco||BD / BBL||Oxoid||Merck|
|Brain Heart Infusion Broth (7116)||237500||211059||CM0225||1.0493|
Brain-Heart Infusion Broth is used for the cultivation of a wide variety of fastidious organisms.
Product Summary and Explanation
Rosenow1 prepared a rich medium for culturing streptococci by combining dextrose broth and brain tissue. Hayden modified the original formula while working with dental pathogens. The current formula is a modification of Rosenow1 and Hayden, using dehydrated infusions of porcine brain and heart tissue.
Brain-Heart Infusion Broth can be supplemented with antibiotics, varying amounts of sodium chloride, yeast extract, and serum to provide a rich medium for bacteria, yeasts and pathogenic fungi. The addition of 0.1% agar can be used to lower oxygen tension, providing an atmosphere to support the growth of aerobic, microaerophilic, and obligate anaerobic microorganisms.
Brain-Heart Infusion Broth, abbreviated as BHI, is specified in many references for food and water testing. NCCLS, National Committee for Clinical Laboratory Standards, cites Brain-Heart Infusion Broth for preparing the inoculum used in antimicrobial susceptibility tests.